CCN Proteins: Methods and Protocols [Paperback]

 This volume provides a comprehensive guide on the Cyr61 (Cysteine-rich 61) (CCN) family of proteins and genes from basic research to cutting-edge methodologies and state-of-the-art techniques. Chapters details practical tips to overcome any obstacles with experimentation pertaining to chemistry, biology, physiology, pathology, medical and dental sciences and pharmacology of CCN proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, CCN Proteins: Methods and Protocols will be a valuable resource for a wide audience, ranging from the experienced CCN researchers looking for new approaches to junior graduate students just beginning in CCN research.

The CCN Proteins: An Overview.- Gene Expression Analysis of CCN Proteins: Whole Mount In Situ Hybridization Of CCN2 in Developing Calcified Tissues .- Expression of CCN Genes and Proteins in Human Skin: Methods And Protocols.- Analysis of Expression of CCN Family Genes in Skeletal Tissue-Derived Cells.- Western Blotting Analysis of CCN Proteins.-  Immunohistochemical Analysis of CCN Proteins.- Analysis of CCN Expression by Immunofluorescence on Skin Cells, Skin, and Reconstructed Epidermis.-  Production of Recombinant CCN2 Protein in Escherichia. Coli.-  Production Of Recombinant CCN Proteins By Brevibacillus Choshinensis.- Production Of Recombinant CCN2 Protein By Mammalian Cells.- In Vitro TransfectiolÃf