Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation [Paperback]

            This volume describes a variety of protocols that will allow the readers to study different aspects of transcriptional and posttranscriptional gene expression regulation in eukaryotic cells. Chapters focus on the latest use of CRISPRi and RNAi technologies for studying various aspects of transcriptional and posttranscriptional regulation and tools to navigate protocols on key bioinformatics.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls

Authoritative and cutting-edge, Eukaryotic Transcription and Post-Transcription Gene Expression Regulation aims to ensure successful results in the further study of this vital field.

1. Fluorescence Reporter-Based Genome-Wide RNA Interference Screening to Identify Alternative Splicing Regulators

Ashish Misra and Michael R. Green

2. Tandem Affinity Purification Approach Coupled To Mass Spectrometry to Identify Post-translational Modifications of Histones Associated with Chromatin-binding Proteins

Sophie Beyer, Philippe Robin, and Slimane Ait-Si-Ali

3. Efficient Preparation of High-Complexity ChIP-Seq Profiles from Early Xenopus Embryos

4. Systematic Discovery Of Chromatin-bound Protein Complexes from ChIP-seq Datasets

Eugenia Giannopoulou and Olivier Elemento

5. Assay for Transposase-Accessible Chromatin with high throughput sequencing (ATAC-seq) Protocol for Zebrafish EmblS.