Proteostasis: Methods and Protocols [Paperback]

This volume highlights the role of proteostasis in human health and associated disease model systems, reflecting its rising importance which has led to the development of new technologies to obtain insight into underling protein mechanistic events. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Proteostasis: Methods and Protocols aims to become a reference book on proteostasis in human health.

UPS Activation in the Battle against Aging and Aggregation-RelatedDiseases: An Extended Review.- ReviewAnd Literature Mining On Proteostasis Factors And Cancer.- Combining Zebrafish and Mouse Models to Test theFunction of Deubiquitinating Enzyme (Dubs) Genes in Development:  Role Of Usp45 in the Retina.-  Immunodepletion and Immunopurification as Approachesfor CSN Research.-  Studying Protein Ubiquitylation in Yeast.- Strategies to Detect Endogenous Ubiquitination of aTarget Mammalian Protein.- In Vitro Ubiquitination  Self-Ubiquitination, ChainFormation, and Substrate Ubiquitination Assays.- Isolation of the Ubiquitin-Proteome From Tumour CellLines and Primary Cells Using Tubes.- Tubes-Mass Spectrometry for Identification andAnalysis of the Ubiquitin-Proteome.- Isolation of Ubiquitinated Proteins to HighPurity  from In Vivo Samples.-  Method for the Purification of Endogenous UnanchoredPolyubiquitin Chains.- Fluorescent Tools for In Vivo Studies onthe Ubiquitin-Proteasome System.- Bimolecular Fluorescence ComplemelsÇ