Wnt Signaling: Methods and Protocols [Paperback]

Now poised to evaluate the potential in modulating Wnt signaling for therapeutic agendas in cancer, wound healing, and degenerative disease, this book collects protocols from an exciting area of study. The methodologies include using Wnt modulating chemicals in engineering tissues from induced pluripotent and embryonic stem cells, monitoring Wnt transcriptional responses in diverse tissues such as bone and skin, and using specific biochemical markers of Wnt signaling to either screen molecular libraries or evaluate novel reagents. These protocols also leverage unique experimental strengths from five different model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

1. Visualizing Wnt Palmiteoylation in Single Cells

Xinxin Gao and Rami N. Hannoush

2. Monitoring Wnt Protein Acylation Using an In Vitro Cyclo-Addition Reaction

Rubina Tuladhar, Nageswari Yarravarapu, and Lawrence Lum

3. Biochemical Methods to Analyze Wnt Protein Secretion

Kathrin Glaeser, Michael Boutros, and Julia Christina Gross

4. Methods for Studying Wnt Protein Modifications/Inactivations by Extracellular Enzymes, Tiki and Notum

Xinjun Zhang and Xi He

5. Probing Wnt Receptor Turnover: A Critical Regulatory Poinls5