Barcoding has proven very useful for correct identification and rapid cataloging of species with less man power and time. Therefore, barcoding is reported in mosquito species found in Western Maharashtra. The book contain following chapters: 1. General introduction 2. Collection methods of mosquitoes 3. Seasonal abundance 4. DNA and RNAs 5. History of molecular evolution 6. DNA sequencing/barcoding 7. Molecular phylogeny of mosquitoes