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This detailed volume describes cutting-edge techniques in three distinct and complementary areas of contemporary kinase biology research. Beginning with a section on synthetic biology, chemical biology, and screening approaches to kinase signaling networks, the book continues with sections on mass spectrometry and metabolic analysis of kinase signaling as well as computational analysis of kinase signaling networks. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
1. Optogenetic Control of Ras/Erk Signaling Using the PhyPIF System
Alexander G. Goglia, Maxwell Z. Wilson, Daniel B. DiGiorno, and Jared E. Toettcher
2. Dissecting Kinase Effector Signaling Using the RapRTAP Methodology
Anne-Marie Ray, Jennifer E. Brinks-Klomp, Kerrie B. Collins, and Andrei V. Karginov
3. Single-Cell Imaging of ERK Signaling Using Fluorescent BiosensorsCopyright © 2018 - 2024 ShopSpell