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Over the past ten years, many powerful new techniques have been developed that have dramatically changed malaria research. The second edition of ?Malaria: Methods and Protocols expands upon the previous edition with current, detailed techniques for laboratory research. With new chapters on parasite culture techniques, genome manipulation methods, 'omic' approaches, and? techniques for studying the biology of the red blood cell and pre-erythrocytic stages of Plasmodium. Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Authoritative and practical, ?Malaria: Methods and Protocols, Second Edition, offers a comprehensive set of standard techniques for laboratory ?researchers.
This Methods in Molecular Biology book expands on the thorough coverage of Malaria its previous edition, offering new chapters on parasite culture methods, genome manipulation and more, and offers materials lists, protocols and troubleshooting tips.Part I. Parasite Culturing
1. In vitro Culturing P. falciparum Erythrocytic Stages
Alexander G. Maier and Melanie Rug
?2. Production of Plasmodium falciparum Gametocytes in vitro
Kathryn Shaw Saliba and Marcelo Jacobs-Lorena
?3. In vitro Differentiation of Plasmodium falciparum Gametocytes into Ookinetes
Anil K. Ghosh and Marcelo Jacobs-Lorena
?4. Ex vivo culture of Plasmodium Vivax and lE
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