Methods in Mouse Atherosclerosis [Hardcover]

This volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive techniques that are widely used both in basic research and in the clinic. The chapters catalogue a wide range of in vitro and in vivo experimental methods used for atherosclerosis research, including thorough protocols and valuable notes based on the authors personal wet lab experience. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Methods in Mouse Atherosclerosis will be a valuable working guide for researchers performing mouse-based atherosclerosis studies.

1. Use of Mouse Models in Atherosclerosis Research

Godfrey S. Getz and Catherine A. Reardon

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2. Cytokines and Immune Responses in Murine Atherosclerosis

P.J.H. Kusters and E. Lutgens

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3. Intravital Microscopy for Atherosclerosis Research

Remco T. A. Megens and Oliver Soehnlein

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4. Non-invasive Molecular Imaging of Mouse Atherosclerosis

Martina A. McAteer and Robin P. Choudhury

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5. Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root

M. Jes?s Andr?s-Manzano, Vicente Andr?s, and Beatriz Dorado

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6. Isolation, Culture, and Polarizatl£§