Mitochondrial Medicine: Volume I, Probing Mitochondrial Function [Hardcover]

This expert volume covers an interdisciplinary and rapidly growing area of biomedical research comprising genetic, biochemical, pathological, and clinical studies aimed at the diagnosis and therapy of human diseases which are either caused by or associated with mitochondrial dysfunction. It dedicates itself to showcasing the tremendous efforts and the progress that has been made over the last decades in developing techniques and protocols for probing, imaging, and manipulating mitochondrial functions. Mitochondrial Medicine: Volume I, Probing Mitochondrial Function focuses on methods being used for the assessment of mitochondrial function under physiological conditions as well as in healthy isolated mitochondria. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Mitochondrial Medicine provides an essential source of know-how and inspiration to all researchers who are fascinated by this tiny organelle that seems so clearly to control the life and death of a single cell and whole organisms alike.

1. Preparation of Functional Mitochondria: A Challenging Business

??????????? Stefan Lehr, Sonja Hartwig, and Jorg Kotzka

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2. Isolation and Quality Control of Functional Mitochondria

??????????? Sonja Hartwig, Jorg Kotzka, and Stefan Lehr

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3. Isolation of Mitochondria-Associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs) from Brain Tissues and Neuronal Cells

??????????? Ida Annunziata, Annette Patterson, and Alessandra dAzzo