Molecular and Cellular Biology of Filamentous Fungi A Practical Approach [Paperback]

The identification and characterization of genes from filamentous fungi has become easier because of the rapid advances that have taken place in molecular biology, including the generation of whole genome sequences. The new challenge facing researchers is to determine the functions of genes and how they contribute to the biology of fungi. To do this it is clear that a wide variety of different experimental techniques will be necessary, ranging from classical genetic analysis and mutant production, right through to cell biology, biochemistry and immunological methods. This book brings together detailed practical guidance from experienced researchers using the full range of these genetic, genomic, cellular and biochemical methods. The authors describe laboratory procedures that have been proven effective for the investigation of a variety of fungal species. They give essential background information, technical tips and detailed experimental protocols, which will prove invaluable to researchers and students studying filamentous fungi.

List of Protocols
Abbreviations
1. Identification and genotypic characterization of filamentous fungi,Michele D. Piercey-Normore and Kieth N. Egger
2. Nucleic acid isolation and analysis,Nicholas J. Talbot
3. DNA-mediated transformation of fungi,Paul Bowyer
4. Genetic analysis ofascomycete fungi,Steven D. Harris
5. Genetic Analysis of basidiomycete fungi,Piers J. Walser et al.
6. Genome analysis in filamentous fungi,Mark L. Farman
7. Cell biology of the duplication cycle in fungi,Michelle Momany
8. Investigations of Oomycete cell biology,Adrienne R. Hardham
9. Signal transduction in filamentous fungi: regulation of protien kinases,Xiang S. Ye et al.
10. Electron microscopy of filamentous fungi,Richard J. O'Connell and Raffaella Carzaniga
11. Biochemical methods for filamentolƒ-