Monoclonal Antibodies in Biotechnology Theoretical and Practical Aspects [Paperback]

This book provides a comprehensive description of principles, methodologies and applications of Monoclonal antibodies to modern science and industry.Monoclonal antibodies are one of the most exciting developments in biotechnology in recent years; this book provides a comprehensive description of principles, methodologies and applications of this powerful technology to modern science and industry.Monoclonal antibodies are one of the most exciting developments in biotechnology in recent years; this book provides a comprehensive description of principles, methodologies and applications of this powerful technology to modern science and industry.This volume provides a complete description of the principles, methodologies and applications of monoclonal antibodies, one of the most exciting developments to occur in biotechnology in recent years, and a powerful technology for modern industry and science. The immune system and the role of the antibody are described and full details are given on how the hybridomas are formed, isolated, and maintained in culture such that the required antibody can be produced to a high degree of purity. The authors describe all the methodologies involved, all the reagents and solutions and all the assay conditions required for their production. The material is presented to enable research and development managers to make choices as to which are the most suitable techniques for their requirements. The book is comprehensively referenced, and will be a necessary resource for all those who are involved with this technology.1. Introduction: the immune response; 2. Making hybridomas (hybridomas technology); 3. Factors affecting successful hybridoma production; 4. Selection of monoclonal antibody-secreting hybridoma cell lines; 5. The large-scale production of monoclonal antibodies in vitro; 6. In conclusion: the diversity in application of monoclonal antibodies and hybridoma technology; References; Index. ...the book provides useful information folsD