Nanoscale Imaging: Methods and Protocols [Hardcover]

This volume presents readers with the latest techniques to study nanoimaging and nanoprobing in application to a broad range of biological systems. The chapters in this book are divided into five parts, and cover topics such as imaging and probing of biomacromolecules including high-speed imaging and probing with AFM; probing chromatin structure with magnetic tweezers; and fluorescence correlation spectroscopy on genomic DNA in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and through, Nanoscale Imaging: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing and expanding field.

Preface&

Table of Contents&

Contributing Authors&

Part I Imaging and Probing of Biomolecules

1.High Resolution Atomic Force Microscopy Imaging of Nucleic Acids

Pablo Ares, Julio Gomez-Herrero, and Fernando Moreno-Herrero

2.Two Ligand Binding Sites in Serotonin Transporter Revealed by Nanopharmacological Force Sensing

Rong Zhu, Hermann J. Gruber, and Peter Hinterdorfer

3.AFM-Based Single-Molecule Force-Spectroscopy of Proteins

Zackary N. Scholl and Piotr E. Marszalek

4.High Resolution AFM-Based Force Spectroscopy

Krishna P. Sigdel, Anna E. Pittman, Tina R. Matin, and Gavin M. King