Neural Tracing Methods: Tracing Neurons and Their Connections [Paperback]

This volume seeks to familiarize readers with a diverse range of technologies and approaches for probing neuron and circuit architecture, and, when possible, to attach detailed protocols to help guide readers toward practical application. From classical lipophilic dye and conjugated lectin tracing techniques, to electrophysiological, in vivo imaging, viral tract tracing, and emerging genetic methods to mark, manipulate, and monitor neural circuits, Neural Tracing Methods: Tracing Neurons and Their Connections includes reference to an arsenal of tools and technologies currently being implemented in model systems ranging from flies to mice. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.

1. A Survey of Current Neuroanatomical Tracing Techniques

??????????? Floris G. Wouterlood

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2. Wheat-Germ Agglutinin (WGA) Tracing: A Classic Approach for Unraveling Neural Circuitry

??????????? Sabrina L. Levy, Joshua J. White, and Roy V. Sillitoe

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3. Retrograde Tract-Tracing Plus: Adding Extra Value to Retrogradely-Traced Neurons

??????????? Jos? L. Lanciego

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4. Simultaneous Collection of In Vivo Functional and Anatomical Data from Individual Neurons in Awake Mice

??????????? Brittany N. Cazakoff and Stephen D. Shea

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5. Single-l3¬