Oogenesis: Methods and Protocols [Paperback]

This volume presents methods and techniques to studyoogenesis in a broad range of organisms, from plants to mammals. Oogenesis: Methods and Protocols guidesreaders through protocols on models ofdevelopmental biology, oogenesis in plants, worms, fruit flies,mosquitos, butterflies, starfish, zebrafish, frog, chicken and mouse. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.

Authoritative and cutting-edge, Oogenesis:Methods and Protocols aims to ensure successful results in the furtherstudy of this vital field.

Histological Analysis of the ArabidopsisGynoecium and Ovules Using Chloral Hydrate Clearing and Differential InterferenceContrast Light Microscopy.- Indirect Immunofluorescence of Proteins in Oogenic Germ Cells of Caenorhabditis Elegans.- Antibody Staining In Drosophila Germaria.- In Vitro<Culturing and Live Imaging of DrosophilaEgg Chambers: A History and Adaptable Method.- Using Fluorescent Reporters to Monitor Autophagy in the Female Germ Line Cellsin Drosophila Melanogaster.- Analysis of Phagocytosis in the Drosophila Ovary.- Immuno-Gold Labeling Of Drosophila FolliclesFor Transmission Electron Microscopy.- Preparation of Drosophila Follicles forTransmission Electron Microscopy.- Quantitative Real-Time PCR Analysis of Gene Transcripts of Mosquito Follicles.- Studying Oogenesis in a Non-Model Organism Using Transcriptomics; Assembling,Annotating,lE