Opioid Receptors: Methods and Protocols [Hardcover]

Opioid Receptors: Methods and Protocols serves as a comprehensive guide to both key new techniques and established methods for the investigation of genetics, structural biology, transcription, and post-transcriptional events of opioid receptors. Other methods cover the cellular detection and trafficking of opioid receptors in vitro and in vivo. Procedures aimed to investigate signaling pathways modulated by opioid receptors and model systems to study opioid receptor-mediated functions are also included. Finally, methods to assay behavioral effects mediated by opioid receptors are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective introductions, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Wide-ranging and authoritative, Opioid Receptors: Methods and Protocols will help both experienced and new entrants in this field to carry out their experiments successfully and with new inspiration.

Part I: Genetic, Structural Biology, Transcriptional and Post-Transcriptional Analysis of Opioid Receptors

?

1. Overview of Genetic Analysis of Human Opioid Receptors

??????????? Santi M. Spampinato

?

2. Computational Structural Biology of Opioid Receptors

??????????? Davide Provasi

?

3. Analysis of Epigenetic Mechanisms Regulating Opioid Receptor Gene Transcription

??????????? Cheol Kyu Hwang, Yadav Wagley, Ping-Yee Law, Li-Na Wei, and Horace H. Loh

?

4. Renilla Luciferase Reporter Assay to Study 3UTR-Driven Post-Transcriptional Regul��