Plant Metabolic Flux Analysis: Methods and Protocols [Hardcover]

In Plant Metabolic Flux Analysis, expert researchers in the field provide detailed experimental procedures for each step of the flux quantification workflow. Steady state and dynamic modeling are considered, as well as recent developments for the reconstruction of metabolic networks and for a predictive modeling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical Plant Metabolic Flux Analysis, seeks to aid scientists in the further study of cutting-edge protocols and methodologies that are crucial to getting ahead in MFA.

This new addition to Springers popular series Methods in Molecular Biology includes stepwise instructions for reproducing cutting-edge techniques in plant metabolic flux analysis, as well as key notes and practical advice from a host of recognized experts.

Martine Dieuaide-Noubhani and Ana Paula Alonso

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2. Metabolic Network Reconstruction and Their Topological Analysis

Marie Beurton-Aimar, Tung Vu-Ngoc Nguyen, and Sophie Colombi?

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Part I: Experimental Design and Acquistion of Labeling Data (Experimental design and data acquisition for isotopic labeling based Metabolic Flux Analysis)?

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3. ¹⁴C pulse Labeling to Estimate External Fluxes and Turnovers in Primary Metabolism

Pianelli Katia, Monier Antoine, Andrieu Marie-H?l?ne, Beauvoit Bertrand, Dieuaide-Noubhani Martine

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4. OptimizatilC=