Plant Pattern Recognition Receptors: Methods and Protocols [Paperback]

This volume covers protocols on techniques ranging from MAMP isolations from diverse microorganisms, PRR identifications from different plant species, MAMP-PRR binding, and a series of signaling responses and events revealed by various biochemical, cellular, genetic and bioinformatic tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Pattern Recognition Receptors: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Peptidoglycan Isolation and Binding Studies with LysM-Type Pattern Recognition Receptors.- Characterization of Plant Cell Wall Damage-associated Molecular Patterns Regulating Immune Responses.- Methods of Isolation and Characterization of Oligogalacturonide Elicitors.- Quantitative Analysis of Ligand-induced Endocytosis of FLAGELLIN-SENSING 2 using Automated Image Segmentation.- Analysis for Protein Glycosylation of Pattern Recognition Receptors in Plants.- Assays to Investigate the N-glycosylation State and Function of Plant Pattern Recognition Receptors.- Steady-State and Kinetics-Based Affinity Determination in Effector-Effector Target Interactions.- In Vitro Ubiquitination Activity Assays in Plant Immune Responses.- Bioinformatics Analysis of the Receptor-Like Kinase (RLK) Superfamily.- Identification of MAPK Substrates using Quantitative Phosphoproteomics.- Analysis of PAMP-Triggered ROS Burst in Plant Immunity.-&nbsplÓ*