Protein and Peptide Analysis by LC-MS Experimental Strategies [Hardcover]

This book is the first example in presenting LC-MS strategies for the analysis of peptides and proteins with detailed information and hints about the needs and problems described from experts on-the-job. The best advantage is -for sure- the practical insight of experienced analysts into their novel protein analysis techniques. Readers starting in 'Proteomics' should be able to repeat each experiment with own equipment and own protein samples, like clean-up, direct protein analysis, after (online) digest, with modifications and others. Furthermore, the reader will learn more about strategies in protein analysis, like quantitative analysis, industrial standards, functional analysis and more.This book is the first its kind of detailed information by LC-MS strategies scanner Peptides and proteins.the most intriguing Advantage is Security of practical Insight of the experienced expertsThis book is the first example in presenting LC-MS strategies for the analysis of peptides and proteins with detailed information and hints about the needs and problems described from experts on-the-job. The best advantage is -for sure- the practical insight of experienced analysts into their novel protein analysis techniques. Readers starting in 'Proteomics' should be able to repeat each experiment with own equipment and own protein samples, like clean-up, direct protein analysis, after (online) digest, with modifications and others. Furthermore, the reader will learn more about strategies in protein analysis, like quantitative analysis, industrial standards, functional analysis and more.Sample Preparation; How to Couple and Handle HPLC and Mass Spectrometry; LC-MS of Proteins; LC-MS of Membrane Proteins; LC-MS of Hydrolyzed Proteins; Online-digest-LC-MS of Proteins; Quantitative LC-MS of Proteins; Top-Down Analysis; LC-MS for the Identification of posttranslational modifications on proteins; Functional Analysis of Proteins by LC-MS; Bioinformatical Tools for the LC-MS Analysis of Proteinslƒ0