Receptor Signal Transduction Protocols: Third Edition [Hardcover]

As our understanding of G protein-coupled receptor (GPCR) signal transduction continues to grow, we cannot help but be struck by the emerging complexity and the ability of this receptor superfamily to continually surprise us as new facets are discovered. In this third edition of Receptor Signal Transduction Protocols, the expert contributors have taken into account the constant evolution of the GPCR field and dealt with methods that allow researchers to address many of the important rising issues.? Serving as a companion to the previous editions, this volume contains thorough revisions of some core chapters, the complete rewriting of others to encompass new technological developments since the publication of the prior editions, and the commissioning of brand new, cutting-edge chapters to expand on previous coverage.? Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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In this third edition of Receptor Signal Transduction Protocols, the expert contributors take into account the constant evolution of the GPCR field. It contains detailed revisions of chapters from the prior editions and a considerable amount of new material.

Part I: Developing Models for Studying GPCRs

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1. Inducible Expression of G Protein-Coupled Receptors in Transfected Cells