RNA Modification [Hardcover]

RNA Modification provides a useful examination of the science and its role in biological regulation, the current frontier of life science research, and includes various RNA modications and their role in gene expression. It represents the most up-to-date knowledge and protocols available today.

• Dynamic RNA modifications and their roles in biological regulation are the current frontier of life science research
• This volume of Methods in Enzymology represents up to date knowledge and protocols

1.?Methodology For The High-Throughput Identification and Characterization of tRNA Variants that are Substrates for a tRNA Decay Pathway
Matthew J. Payea, Michael P. Guy, and Eric M. Phizicky
2.?Nucleoside Analysis by Hydrophilic Interaction Liquid Chromatography Coupled with Mass Spectrometry
Yuriko Sakaguchi, Kenjyo Miyauchi, Byeong-il Kang, and Tsutomu Suzuki
3.?A Platform for Discovery and Quantification of Modified Ribonucleosides in RNA: Application to Stress-Induced Reprogramming of tRNA Modifications
Weiling Maggie Cai, Yok Hian Chionh, Fabian Hia, Chen Gu, Stefanie Kellner, Megan E. McBee, Chee Sheng Ng, Yan Ling Joy Pang, Erin G. Prestwich, Kok Seong Lim, I. Ramesh Babu, Thomas J. Begley and Peter C. Dedon
4.?Recognition of Specified RNA Modifications by the Innate Immune System
Tatjana Eigenbrod, Patrick Keller, Steffen Kaiser, Katharina Rimbach, Alexander H. Dalpke and Mark Helm
5.?Kinetic Analysis of tRNA Methyl Transferases
Ya-Ming Hou and Isao Masuda
6.?Preparation of Human Nuclear RNA m6A Methyltransferases and Demethylases and Biochemical Characterization of Their Catalytic Activity
Jianzhao Liu, Yanan Yue, and Chuan He
7.?Transcriptome-Wide Mapping of N6-Methyladenosine by m6A-Seq
Dan Dominissini, Sharon Moshitch Moshkovitz, Ninlc+