Salamanders in Regeneration Research Methods and Protocols [Hardcover]

This detailed volume focuses on best practices and conditions for maintaining the most commonly used salamander species in the laboratory. Salamanders in Regeneration Research: Methods and Protocols guides readers through experimental manipulations in vivo and in vitro, respectively. With methods on targeting a wide variety of structures, ranging from the limb to the heart and to the brain, and methods for studying genetically modified organisms and tools for mining in the genomic databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Salamanders in Regeneration Research: Methods and Protocols provides a comprehensive collection of methods chapters.

Part I: Salamanders

1. Variation in Salamanders: An Essay on Genomes, Development, and Evolution

Jeremy P Brockes

?2. Maintaining Eastern Newts (Notophthalmus viridescens) for Regeneration Research

Hans-Georg Simon and Shannon Odelberg

3. Housing and Maintenance of Ambystoma mexicanum, the Mexican Axolotl

Johanna E. Farkas and James R. Monaghan

?4. Husbandry of Spanish Ribbed Newts (Pleurodeles waltl)

Alberto Joven, Matthew Kirkham, and Andr?s Simon

?5. Maintaining Plethodontid Salamanders in the Laboratory for Regeneration Studies

Claudia Marcela Arenas G, Andrea G?mez-Molina, and? Jean Paul Delgado

?Part II: Experimental Manipulation in Salamanders

?6. Newt Lens Transdifferentiation: From Lentectomy to Immuno-FISH