Semaphorin Signaling: Methods and Protocols [Hardcover]

This volume arranged into three sections describes biochemical, in vitro, and in vivo protocols on Semaphorins. Chapters focus on approaches that would allow the novice to study Semaphorins and employ robust assays to characterize mechanisms of action. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

1. Semaphorins and their Signaling Mechanisms

Laura T. Alto and Jonathan R. Terman

 

Part I: Biochemical Approaches

2. Isolating Fc-Tagged SEMA4D Recombinant Protein from 293FT Cells

Joanne Soong and Glynis A. Scott

 

3. Expression and Purification of Class 7 Semaphorin and its Plexinc1 Receptor Using Baculovirus-Mediated Mammalian Cell Gene Transduction

 

4. Immunoaffinity Purification of the Glycosylated Extracellular Fragment of Mouse Plexin A2 Produced in a Mammalian Expression System

Terukazu Nogi, Emiko Mihara, Norihisa Yasui, and Junichi Takagi

5. Plate-Based Assay for Measuring Direct Semaphorin-Neuropilin Interactions

Matthew W. Parker and Craig W. Vander Kooi

 

6. Characterizing Plexin Gtpase InteractilóT