Thyroid Hormone Nuclear Receptor Methods and Protocols [Hardcover]

This volume aims to detail the advances in the field of Thyroid hormones (THs), namely T3 and T4, and their nuclear receptor TRs from a conceptual and methodological point of view. Chapters guide readers through a general introduction, TR's engineered mice, human genetics of TRs, control of the hypothalamus-pituitary-thyroid (HPT) axis, the relevance of the local THs production and metabolism and pathological consequences of TH/TR alterations. Non-mammalian models are also presented and discussed. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Thyroid Hormone Nuclear Receptor: Methods and Protocols aims to ensure successful results in the further study of this vital field.

1. Thyroid Hormone Receptors: Several Players for One Hormone and Multiple Functions

Jacques Samarut and Michelina Plateroti

 

2. Two Protocols to Study the Interactions of Thyroid Hormone Receptors with Other Proteins and Chromatin

Wenyue Guan, Romain Guyot, and Fr?d?ric Flamant

 

3. Expression Analysis of Genes Regulated by Thyroid Hormone in Neural Cells

Juan Bernal and Beatriz Morte

4. In vitro Approaches to Identify Thyroid Hormone Receptor-dependent Transcriptional Response

Maria Sirakov and Michelina Plateroti

 

5. Posttranslational Modification of Thyroid Hormone Nuclear Receptor by Phosphorylation