Translational Regulation of Gene Expression [Paperback]

1 Translational Regulation of Ribosomal Proteins in Escherichia coli: Molecular Mechanisms.- 1. Evidence for Autoregulation of Ribosomal Proteins.- 2. Regulation of the ? Operon by S4.- 2.1. Introduction.- 2.2. Thermodynamics of S4-Messenger RNA Complex Formation.- 2.3. Structure of the ? Messenger RNA Leader.- 3. Regulation of the L11 Operon by L1.- 4. Regulation of the rif Operon by L10.- 5. Other Ribosomal-Protein Repressors.- 6. Common Themes in Ribosomal-Protein Autoregulation.- 7. Thermodynamics of Translational Repression.- 7.1. Thermodynamics of Translation.- 7.2. Translation and Repression In Vivo.- 8. Predictions of Different Translational Repression Models.- 8.1. Displacement Model.- 8.2. Entrapment Model.- 8.3. Influences of Protein Binding on Messenger RNA Turnover...- 8.4. Prediction of Gene-Dosage Effects.- 9. Influence of Messenger RNA Secondary Structure on Translation...- 10. Future Directions.- References.- 2 Translational Regulation in Bacteriophages.- 1. Introduction.- 2. RNA Phage.- 2.1. Repression by Replicase.- 2.2. Repression by Coat Protein.- 3. T4 Gene 32.- 3.1. Autogenous Translational Repression.- 3.2. Binding Parameters.- 3.3. Quantitative Model of Repression.- 3.4. Tests of the Model.- 4. f1 Gene V.- 5. P22 Gene 8.- 6. T4 RegA Protein.- 7. Structural Repression and Activation.- 8. Conclusions.- References.- 3 Escherichia coli Threonyl-Transfer RNA Synthetase as a Model System to Study Translational Autoregulation in Prokaryotes.- 1. Introduction.- 2. Structure of the Escherichia coli Genome around the Gene for Threonyl-Transfer RNA Synthetase.- 3. The Expression of the Gene for Threonyl-Transfer RNA Synthetase Is Negatively Autoregulated at the Translational Level.- 3.1. In Vitro Studies.- 3.2. In Vivo Studies.- 4. Genetic Definition of the Translational Operator.- 4.1. Isolation of Operator Constitutive Mutants.- 4.2. Nucleotide Sequence of the Operator Constitutive Mutants.- 4.3. Homologies between the thrS Translational Operator andlS!