Vaccines for Invasive Fungal Infections: Methods and Protocols [Paperback]

This detailed volume focuses on the development of mycosis vaccines via the most common etiological agents of mycoses, such as Aspergillus and Candida species, followed by Cryptococcus, dimorphic fungi, and members of Mucoraceae family of fungi. The book is sectioned into five parts that describe experimental approaches for vaccine candidate discovery, improved vaccine design and delivery, assessment of critical endpoints, analysis of mechanisms of mycosis vaccine protection, and key methods for clinical translation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting known pitfalls. 

Part I: Discovery and Testing of Vaccine Candidates

 

1. Mouse Immunization with Radioattenuated Yeast Cells of Paracoccidioides brasiliensis

            Estef?nia Mara do Nascimento Martins and Antero Silva Ribeiro de Andrade

 

2. Heat-Killed Yeast as a Pan-Fungal Vaccine

            Marifelc)