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This detailed volume includes a rich variety ofapplications using various instrumentations, probes, disease models, andtargets in order to account for the multidisciplinary nature of the use of invivo fluorescence imagine. The book also includes chapters on the emergingfields of cell tracking, image-guided treatment, and fluorescence imaging inthe second NIR window, as well as protocols for evaluation methods before andafter in vivo imaging. Written for the highly successful Methods in Molecular Biology series, chapters include briefintroductions to their respective topics, lists of the necessary materials andreagents, step-by-step readily reproducible laboratory protocols, and tips ontroubleshooting and avoiding known pitfalls.
1. Before In VivoImaging: Evaluation of Fluorescent Probes using Fluorescence Microscopy,Multiplate Reader, and Cytotoxicity Assays
ShaojuanZhang
2. BioconjugationMethods for Coupling Targeting Ligands with Fluorescent Dyes
Xiaoxi Ling
3. In VivoFluorescence Reflectance Imaging with Subcutaneous Mouse Tumor Models
Jie Cao andMingzhou Zhou
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