Xenopus: Methods and Protocols [Hardcover]

This volume explores techniques used to study and experiment with Xenopus in order to model or understand human disease. The chapters in this book cover topics such as implementation of CRISPR/Cas9 and TALEN in Xenopus from the design stage up to the genotyping stage; methods to explore generation of knock-in animals; cancer modelling and in vivo screening of congenital heart disease; electroporation; and phenotyping at the organismal level, cellular level, and proteome level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Xenopus: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this wide and developing field. This book will aid in keeping Xenopus at the forefront for biochemical, cell biological, and developmental studies and forward it as a preclinical disease model.

Preface&

Table of Contents&

Contributing Authors&

1. Husbandry, General Care, and Transportation of Xenopus laevis, and Xenopus tropicalis

Sean McNamara, Marcin Wlizla, and Marko E. Horb

2. Generation and Care of Xenopus laevis and Xenopus tropcalis Embryos

Marcin Wlizla, Sean McNamara, and Marko E. Horb

3. Methods for CRISPR/Cas9 Xenopus tropicalis Tissue-Specific Multiplex Genome Engineering

Thomas Naert anl£Ý