Xylem: Methods and Protocols [Paperback]

This volume provides detailed techniques used for the study and characterization of the plant vascular system, with a central focus on the xylem tissue.  Chapters are organized in three main sections covering; analysis of xylem development, xylem characterization though imaging techniques, and analysis of the xylem composition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical Xylem: Methods and Protocols, aims to ensure successful results in the further study of this vital field.

Part I: DEVELOPMENT

 

1 Experimental and Theoretical Methods to Investigate Vascular Development in Plants

 

2 Strigolactone-Mediated Stimulation of Secondary Xylem Proliferation in Stems

Javier Agust?

 

3 Quick Histochemical Staining Methods to Detect Cell Death in Xylem Elements of Plant Tissues

Sacha Escamez, Benjamin Bollh?ner, and Hannele Tuominen

 

4 Establishment and Utilization of Habituated Cell Suspension Cultures for Hormone-Inducible Xylogenesis

Delphine M?nard, Henrik Serk, Rapha?l Decou, and Edouard Pesquet

 

5 Tissue Culture for Xylem Differentiation with Arabidopsis Leaves

Masato Saito, Alif Meem Nurani, Yuki Kondo, and Hiroo Fukuda

 

6 VND6-Induced Xylem Cell Differentiation in Arabidopsis Celll��