The Zebrafish Genetics, Genomics, and Transcriptomics [Hardcover]

The Zebrafish: Genetics, Genomics, and Transcriptomics, Fourth Edition, is the latest volume in the Methods in Cell Biology series that looks at methods for the analysis of genetics, genomics, and transcriptomics of Zebrafish.

Chapters cover such topics as gene-trap mutagenesis, genetic Screens for mutations, gene editing in zebrafish, homologous gene targeting, genome-wide RNA tomography, and developmental epigenetics and the zebrafish interactome.

• Covers sections on model systems and functional studies, imaging-based approaches, and emerging studies
• Presents chapters written by experts in the field
• Contains cutting-edge material on the topic

Part 1. Forward and Reverse Genetics 1. Multiplex Conditional Mutagenesis in Zebrafish Using the CRISPR/Cas System 2. Tol2-Mediated Transgenesis, Gene Trapping and Enhancer Trapping, and the Gal4-UAS System 3. Genetic Screens for Mutations Affecting Adult Traits and Parental-Effect Genes 4. Targeted Candidate Gene Screens Using CRISPR/Cas9 Technology 5. TALEN and CRISPR Enhanced DNA Homologous Recombination for Gene Editing in Zebrafish 6. Precise Genome Editing by Homologous Recombination 7. The Zebrafish Genome Editing Toolkit 8. Clonal Analysis of Gene Loss-of-function and Tissue-specific Gene Deletion in Zebrafish via CRISPR/Cas9 Technology 9. Tissue-Specific Gene Targeting Using CRISPR/Cas9

Part 2. Transgenesis and Functional Genomics 10. Transcriptional Regulation Using the Q System in Transgenic Zebrafish 11. Contemporary Zebrafish Transgenesis with Tol2 and Application for Cre/lox Recombination Experiments 12. Method? for Somatic Cell Nuclear Transfer in Zebrafish 13. Experimental Approaches to Studying the Nature and Impact of Splicing Variation in Zebrafish 14. PICCORO: A Techlã€